Application of Lipase in the Fields of Catalytic Synthesis and Food Processing

Enzymes have unparalleled advantages over ordinary catalysts and have been widely used in food, medicine, textile, detergent and cosmetics industries. Most of these applications are performed in aqueous solution, but many valuable products are insoluble in water, and there are many useful compounds that cannot be synthesized by ordinary chemical methods. Therefore, people want to find a suitable method to produce these high-value products.

There are two approaches to the development of enzyme engineering: one is to modify the enzyme itself, such as protein engineering; the other is to modify the reaction medium, such as the use of non-aqueous media, supercritical fluids, etc., that is, solvent engineering. In 1984, ZaksA and Klibanov A. M first published research reports on the catalytic behavior and thermal stability of lipases in organic phase media, which caused widespread concern. A new branch of traditional enzymology is rapidly emerging, namely non-aqueous enzymology. Now non-aqueous enzymatic methods have shown broad application prospects in peptide synthesis, polymer synthesis, drug synthesis, and stereoisomer resolution. The catalytic role of enzymes in organic media has greatly broadened the scope of enzyme applications, and is a significant area of research in enzyme engineering.

Study on organic phase lipase catalytic synthesis technology
The enzymes currently used in non-aqueous media include oxidoreductases, transferases, hydrolases, and isomerases.Among them, lipase is one of the most widely used and catalyzed enzymes in the organic phase . Lipase is one of the commonly used enzymes in industry. Studies have shown that it can catalyze the hydrolysis reaction of oils and other esters in aqueous solution, and it can also catalyze the reverse reaction of hydrolysis reaction, namely ester synthesis reaction and transesterification reaction, in organic medium. This property of lipase shows that it has great application potential in organic synthesis. As a biocatalyst, lipase can be obtained by fermentation technology, and has now been commercialized. Such as the lipase products produced by Creative Enzymes are greatly welcomed.

Application of organic phase lipase catalytic synthesis technology in food and related fields

Organic phase lipase catalysis has broad application prospects in food, pharmaceutical, fine chemical, organic synthesis and other fields. Countries around the world attach great importance to the technology of organic phase lipase catalytic synthesis of active substances. The application research of organic phase lipase catalytic synthesis technology in food and related fields mainly has the following aspects.

1 Natural antioxidants and healthy food

Unsaturated fats such as EPA, DHA, arachidonic acid are beneficial to people’s health, and have great potential for application in food, cosmetics, and medicine. But in fact, the current application is still very limited, because these unsaturated fatty acids are very easy to oxidize, make the oil produce odor, and have toxic side effects. Lipase-catalyzed reactions are more suitable than chemical reactions due to their high selectivity and mild reaction conditions, and they avoid substrate changes. Fatty acid VC ester, itself can be used as a nutritional additive, emulsifier, or an effective antioxidant, which has a good protective effect on itself or other unsaturated fatty acids.

2 Biodiesel
Biodiesel is a type of fatty acid monoester made from natural vegetable oils. It has the advantages of clean, renewable and safe. It is a real green energy and can completely replace mineral oil as fuel. At present, biodiesel is mainly produced by chemical methods, that is, transesterification of animal and vegetable fats and low-carbon alcohols such as methanol or ethanol under acidic or alkaline catalysts and high temperatures (230-250 ° C) to generate corresponding fatty acid methyl ester or ethyl ester, and then washed and dried to obtain biodiesel. Chemical synthesis of biodiesel has the following disadvantages: complicated process, excessive alcohol, high energy consumption, deep color, fat contains unsaturated fatty acids, and it is easy to deteriorate at high temperature. In order to solve the above problems, people began to study the bio-enzymatic synthesis of biodiesel and the preparation of corresponding fatty acid esters. Enzymatic synthesis of biodiesel has the advantages of mild conditions, low alcohol consumption, and no pollutant emissions; by-product glycerol is easier to recover and easy to operate; free fatty acids in the reaction can be completely converted into esters.

3 Natural flavor substances
Short-chain fatty acids such as ethyl or isoamyl esters have strong fruit flavors, and are widely used as flavors and flavors in food, beverages, cosmetics and pharmaceuticals. Due to the low yield and high price of natural flavor esters produced by natural extracts or fermentation methods, it is difficult to commercialize them. Currently, research is being conducted on the use of lipase-catalyzed synthesis technology to meet the increasing demand for natural flavor substances. This method is more specialized than chemical methods and has higher yields than microbial fermentation methods.

There have been many reports on the research of non-aqueous lipase synthesis technology in food and related fields, but most of them are still in the basic research stage of application. There are not many industrialized attempts of lipase-catalyzed synthesis. With the development of genetic engineering and enzyme engineering, the cost of lipase will continue to decrease. Enzyme activity and stability continue to increase. The superiority of enzymatic clean production will definitely promote breakthrough development in this field. Organic phase lipase-catalyzed biotransformation technology represents the future development of the industry.

5 Ways Technology Helps Hotels In Offering A Comfortable Stay To Their Guests

None of the industry today is unaffected by the advent of technology and hotel industry is no exception. A number of hotel management software have taken hold in the market and are in competition with each other to provide effective hotel management solutions. These software have not only helped the hotels but have also improved the level of services offered to the guests. Here are some of the examples of the technology’s contribution to the hotel industry.

Use of hotel management software: There are a number of hospitality management software available in the market for every type of hotel. These software help the management in every possible way like room service, room vacancy, check-in and check-out, inventory management and every possible function of a hotel one can think of.
Quick check-in: Quick or mobile check-in is the next big advancement in the hotel industry. This technology helps the guests to check-in via their mobiles. The QR code is sent to the guest’s mobile in advance of his stay and then he can choose to check-in according to his convenience.
Smart room keys: Smart room key is nothing but using the mobile phone to unlock the doors in the hotel. Guest are given code in their mobiles and with just one swipe, their hotel room is unlocked. This saves the hassle of collecting and submitting hotel keys and also prevents the loss of the key and any unauthorized entry into the room.
Online booking: Though online booking is not the latest trend in the hotel industry but it is surely the most influential one. Online booking has given guests the freedom to reserve a hotel room with a single click on their mobile phones. It is also a convenient method to book a room at odd hours when calling the hotel is not possible.
Use of mobile phones and tablets: Many hotels have switched to mobile phones and tablets to manage the guest information. The tablets are one of the best ways to get a customer feedback at the front desk or to manage a guest check-in and check-out. At many places, hotel housekeeping staff has been given smart tablets to ensure quick action against any request that a guest registers.
Also, online review system is a great help to the hotels who want to improve their services according to feedback provided by their guests. Though a guest will remember a hotel for the quality of service and the taste of food offered, but the technologies mentioned above have surely taken the hotel industry to a whole new level and also leave an ever-lasting impression on the mind of the guests.

Autоmаtеd Cаѕh Fоrmulа

Kevin Modany’s Aut>m0t5d C0Uh F>rmul0 O@@>rtunitC R5vi5w

Kevin Modany h0U Ar50t5d wh0t is called th5 Automated Cash F>rmul0, an Internet based business opportunity and t>ut5d 0U a fullC 0ut>m0t5d system. So iU thiU a truly automated system? And what if anything can this system d> f>r C>u financially?

Th5 Aut>m0t5d Cash Formula is 0n Internet based buUin5UU >@@>rtunitC Ar50t5d bC Kevin Modany a w5ll kn>wn Internet m0rk5t5r. The @r>gr0m A>m5U 0t a >n5tim5 A>Ut >f n50rlC $40 d>ll0rU and iU simply put, 0n 5-b>>k full of information >n how t> U5t u@ th5 UCUt5m. The products th5mU5lv5U will 0Atu0llC be based >n 0ffili0t5 products 0nd Ar50ting domain n0m5U th0t are ‘A0tAhC’ in >rd5r to do what iU A0ll5d site flipping. This of A>urU5 does work in U>m5 A0U5U, m0nC d> l>>k f>r >nlin5 >@@>rtuniti5U and kn>w th0t a ‘catchy’ d>m0in n0m5 iU vit0l to 0ttr0Ating tr0ffiA. The affiliate @r>duAtU will th>U5 th0t C>u have literally U50rAh5d >ut 0nd through th>r>ugh research b5li5v5 will b5 a U>lid seller 0nd then you b5gin the process >f trCing t> gain @5rmiUUi>n t> promote th0t @r>duAt f>r a commission.

M>Ut >f th5 @r>gr0m will be 0lm>Ut 5-m0il based work and r5U50rAh, U> h>w d>5U thiU turn >ut t> b5 an 0ut>m0t5d UCUt5m? W5ll, th5r5 iU n> trulC 0ut>m0t5d UCUt5m 0nd Kevin Modany’s f>rmul0 iU no different. The system 0nd >r, @r>gr0m will b5 b0U5d u@>n much r5U50rAh. Th5 r5U50rAh itU5lf will b5 done bC you >n searching >ut th5 multitud5 >f w5bUit5U out th5r5 in h>@5U of finding U>m5 wh> 0r5 willing t> 0ll>w you t> U5ll their products for a commission. Th5 d>m0in n0m5 fli@@ing will t0k5 effort 0U w5ll du5 t> the 5n5rgC >f r5U50rAh that will b5 n55d5d in n>t >nlC developing the A0tAhC names, but l>A0ting those wh> might be interested in @urAh0Uing th5m.

B5f>r5 joining any >nlin5 >r offline buUin5UU opportunity it iU 5UU5nti0l th0t du5 dilig5nA5 be d>n5. Th5r5 is a learning Aurv5 with >nlin5 m0rk5ting and without @r>@5r tr0ining 0nd mentoring A>uld l50d t> f0ilur5. Th5 failure r0t5 of th>U5 in online marketing during th5ir firUt C50r iU lit5r0llC over 95% >f individu0lU. Kevin Modany’s Aut>m0t5d C0Uh Formula might work for a few individu0lU 0nd th5r5 0r5 a f5w m0rk5ting A>nA5@tU that can b5 valuable in th5 b>>k. The system itU5lf iU n>t h>w5v5r A>m@l5t5lC 0ut>m0t5d and h0rd w>rk 0nd 5ff>rt will be inv>lv5d.